skip to content
Fundamentals of Enzyme Kinetics. Preview this item
ClosePreview this item

Fundamentals of Enzyme Kinetics.

Author: Athel Cornish-Bowden
Publisher: Weinheim : Wiley, 2013.
Edition/Format:   eBook : Document : English : 4th edView all editions and formats
Summary:
Now in its fourth edition, this textbook is one of the few titles worldwide to cover enzyme kinetics in its entire scope and the only one to include its implications for bioinformatics and systems biology. Multi-enzyme complexes and cooperativity are therefore treated in more detail than in any other textbook on the market. The respected and well known author is one of the most experienced researchers into the topic  Read more...
Getting this item's online copy... Getting this item's online copy...

Find a copy in the library

Getting this item's location and availability... Getting this item's location and availability...

WorldCat

Find it in libraries globally
Worldwide libraries own this item

Details

Genre/Form: Electronic books
Additional Physical Format: Print version:
Cornish-Bowden, Athel.
Fundamentals of Enzyme Kinetics.
Weinheim : Wiley, ©2013
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Athel Cornish-Bowden
ISBN: 9783527665488 352766548X 9783527330744 3527330747 9781299241718 1299241719
OCLC Number: 830166806
Notes: 5.5 Modifications to the King-Altman method.
Description: 1 online resource (498 pages)
Contents: Title Page; Contents; 1 Basic Principles of Chemical Kinetics; 1.1 Symbols, terminology and abbreviations; 1.2 Order of a reaction; 1.2.1 Order and molecularity; 1.2.2 First-order kinetics; 1.2.3 Second-order kinetics; 1.2.4 Third-order kinetics; 1.2.5 Zero-order kinetics; 1.2.6 Determination of the order of a reaction; 1.3 Dimensions of rate constants; 1.4 Reversible reactions; 1.5 Determination of first-order rate constants; 1.7 Catalysis; 1.8 The influence of temperature and pressure on rate constants; 1.8.1 The Arrhenius equation; 1.8.2 Elementary collision theory. 1.8.3 Transition-state theory1.8.4 Effects of hydrostatic pressure; Chapter summary; Problems; 2 Introduction to Enzyme Kinetics; 2.1 The idea of an enzyme-substrate complex; 2.2 The Michaelis-Menten equation; 2.3 The steady state of an enzyme-catalyzed reaction; 2.3.1 The Briggs-Haldane treatment; 2.3.2 Parameters of the Michaelis-Menten equation; 2.3.3 Units of enzyme activity; 2.3.4 The curve defined by the Michaelis-Menten equation; 2.3.5 Mutual depletion kinetics; 2.3.6 Ways of writing the Michaelis-Menten equation; 2.4 Specificity; 2.4.1 The fundamental property of enzymes. 2.4.2 Discrimination between mixed substrates2.4.3 Comparing different catalysts; 2.5 Validity of the steady-state assumption; 2.6 Graphs of the Michaelis-Menten equation; 2.6.1 Plotting v against a; 2.6.2 The double-reciprocal plot; 2.6.3 The plot of a/v against a; 2.6.4 The plot of v against v/a; 2.6.5 Origins of the plots; 2.6.6 The direct linear plot; 2.7 The reversible Michaelis-Menten mechanism; 2.7.1 The reversible rate equation; 2.7.2 The Haldane relationship; 2.7.3 "One-way enzymes"; 2.8 Product inhibition; 2.9 Integration of enzyme rate equations. 2.9.1 Michaelis-Menten equation without product inhibition2.9.2 Effect of product inhibition on progress curves; 2.9.3 Other problems with time courses; 2.9.4 Characterizing mutant enzymes; 2.9.5 Accurate estimation of initial rates; 2.9.6 Time courses for other mechanisms; Chapter summary; Problems; 3 "Alternative" enzymes; 3.1 Introduction; 3.2 Artificial enzymes; 3.3 Site-directed mutagenesis; 3.4 Chemical mimics of enzyme catalysis; 3.5 Catalytic RNA; 3.6 Catalytic antibodies; Chapter summary; Problems; 4 Practical Aspects of Kinetics; 4.1 Enzyme assays. 4.1.1 Discontinuous and continuous assays4.1.2 Estimating the initial rate; 4.1.3 Increasing the straightness of the progress curve; 4.1.4 Coupled assays; 4.2 Detecting enzyme inactivation; 4.3 Experimental design; 4.3.1 Choice of substrate concentrations; 4.3.2 Choice of pH, temperature and other conditions; 4.3.3 Use of replicate observations; 4.4 Treatment of ionic equilibria; Chapter summary; Problems; 5 Deriving Steady-state Rate Equations; 5.1 Introduction; 5.2 The principle of the King-Altman method; 5.3 The method of King and Altman; 5.4 The method of Wong and Hanes.

Abstract:

Now in its fourth edition, this textbook is one of the few titles worldwide to cover enzyme kinetics in its entire scope and the only one to include its implications for bioinformatics and systems biology. Multi-enzyme complexes and cooperativity are therefore treated in more detail than in any other textbook on the market. The respected and well known author is one of the most experienced researchers into the topic and writes with outstanding style and didactic clarity. As with the previous editions, he presents here steady-state kinetics and fast reactions, supplementing each chapter wi.
Retrieving notes about this item Retrieving notes about this item

Reviews

User-contributed reviews

Tags

Be the first.

Similar Items

Related Subjects:(3)

Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.